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KMID : 0357920050390030168
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Korean Journal of Pathology
2005 Volume.39 No. 3 p.168 ~ p.171
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Mutational Analysis of Proapoptotic bcl-2 Family genes in Colon Carcinomas
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Song Young-Hwa
Nam Suk-Woo
Yoo Nam-Jin
Park Won-Sang
Lee Sug-Hyung
Kim Su-Young
Lee Jong-Woo
Lee Jung-Young
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Abstract
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Background: Several lines of evidence have indicated that the deregulation of apoptosis is involved in the mechanisms of cancer development, and somatic mutations of the apoptosis-related genes have been reported in human cancers. Members of the bcl-2 family proteins regulate the intrinsic apoptosis pathway mainly in the mitochondria. The aim of this study was to explore whether the somatic mutation of the proapoptotic bcl-2 family genes, one of the mechanisms that prolong the survival of cancer cells, occurred in colorectal carcinomas.
Methods: In the current study, to detect the somatic mutations in the DNA sequences encoding the bcl-2 homology 3 (BH3) domain of the human bak, bid, bik, bim, PUMA, bcl-rambo, bcl-G, and bmf genes in 98 colon adenocarcinomas, we used polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), and DNA sequencing.
Results: The SSCP analysis detected no evidence of somatic mutations of the genes in the coding regions of the BH3 domain in the cancers.
Conclusions: The data presented here indicate that the proapoptotic bcl-2 family genes, bak, bid, bik, bim, PUMA, bcl-rambo, bcl-G and bmf may not be somatically mutated in human colorectal carcinomas, and suggest that the colorectal cancers may not utilize mutational events of these proapoptotic bcl-2 family genes in the mechanisms for evading apoptosis.
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KEYWORD
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Colonic neoplasms, Genes, bcl-2, Apoptosis, Mutation
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